Effects of the Nd: YAG Laser on In vitro Fibroblast Attachment to EndotoxinTreated Root Surfaces

摘要

The purpose of this thesis was to evaluate the effects of the Nd:YAG laser on invitro fibroblast attachment to endotoxin treated root surfaces and to describe any root surface alterations resulting from the use of the laser. Thirty 4 mm x 4 mm cementum segments were obtained from unerupted third molars. Treatment groups were assigned as follows: (1) non-lased, non-diseased; (2) nonlased, diseased; and (3) lased, diseased. Each group contained ten root segments. The roots were considered diseased after soaking them in endotoxin (E. coli 055:b5 lipopolysaccharide (556 EU/ml)) for 72 hours. The lased, diseased root segments were treated with a neodymium: yttrium aluminum garnet (Nd:YAG) laser using a 320 micrometer contact optic fiber handpiece with an energy setting of 80 mJ at 10 pulses per second for one minute. The fiber was held perpendicular to the root surface. An attempt was made to cover the entire root surface equally during the one minute of exposure.