Detection of Threshold Numbers of Escherichia coli and Pseudomonas aeruginosaUsing a Hand-Held Detection Device

摘要

Minimum colony forming units per milliliter (CFU/mL) of Escherichia coli (E.coli) and Pseudomonas aeruginosa (Ps. aeruginosa) were determined by the bioluminescent generation of light which was monitored in a hand-held detection device, the Monolight 100. Light energy resulted from the interaction of luciferin and adenosine triphosphate (ATP) in the presence of luciferase and magnesium. E. coli and Ps. aeruginosa were detectable at 1 x 103 CFU/mL and 3.6 X 103 CFU/mL, respectively. The Monolight 100 was useful for detection but required tedious and meticulous techniques, difficult to execute in the field.