Study of the Utility of Lipopolysaccharide Binding Protein for Diagnosis andTreatment of Endotoxemia

摘要

We have defined the interactions of LPS binding protein (LBP) with variousisolates of bacterial endotoxin (lipopolysaccharide, LPS) or lipid A partial structures. Our studies include direct binding measurements and evaluation of the biologic activity of LPS-LBP complexes. These structure/function studies demonstrate the utility of LBP as a detecting reagent for an assay to measure LPS in biologic fluids. To facilitate this we have also developed the methods to prepare large quantities of recombinant LBP and have produced monoclonal antibodies to human LBP. All of the requisite reagents and basic information are available to facilitate development of a clinically useful assay for LPS. The major limitation in this effort is the termination of funding of this project.... Gram-negative bacterial endotoxin, Lipopolysaccharide, Septic shock, ARDS, Endotoxin assay, Macrophage, Monocyte, Cytokine, Multi-organ failure, RA 2.