Transfection of Murine and Human Hematopoietic Progenitors with RearrangedImmunoglobulin Genes

摘要

There are three main objectives of the research conducted under the abovecontract. First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to be activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig-genes into human hematopoietic progenitors then grow and activate those cells in an animal model.