Convection-Enhanced Delivery (CED) in an Animal Model of Malignant Peripheral Nerve Sheath Tumors and Plexiform Neurofibromas.

摘要

Our experiments for the current study are ongoing. Unfortunately, work will not be completed within the time frame of the DOD grant. We have encountered several challenges in completing this. Work on this project was initially delayed by several months secondary to difficulty in obtaining regulatory approval from both our own IACUC and the DOD. Additionally, though we were able to establish and maintain an MPNST cell line suitable for our needs, we have been unable to maintain a cell line for benign plexiform neurofibroma. We have not been able to successfully establish the xenograft within the sciatic nerve. Using the MPNST cell line we were able to perform in vitro studies, comparing efficacy of erlotinib, rapamycin and imatinib in inhibition of cell proliferation and established that erlotinib is the most potent inhibitor for this particular cell line. The cell line we are using is aggressive and grows rapidly as a flank xenograft though we have not been able to successfully establish the xenograft within the sciatic nerve.