Mosaic structures of neurotoxins produced from Clostridium botulinum types C and D organisms

摘要

We isolated the gene encoding a holulinnm neumloxin (BoNT) of 12H5 amino acids with a molecular weight of 147 364 from the toxigenic baelcriophage d sA nl Clnxiritliuin botitlinwn type D strain South African (Dsa). The BoNT of Dsa (BoNT/D.sa) i.s composed of three regions mi the basis of the limnology to BoNT types Cl (BoNT/Cl) and D (BoNT/D). The N-terminal (Met-1 to Val-522) and ihe C-tenninal regions (Tip 'MS in (11111285) have high identity to corresponding regions of BoNT/D (96% identity) and BoNT/Cl (74% identity), respectively. The core region (I'm-523 to lys-M4) is common to three toxins (83% to 92% identity). These results suggest that neurotoxins produced from ("histritliiitii hotiiliiuini types C and D are composed in a mosaic-like fashion.