Male sterility of triticale lines generated through recombination of triticale and rye maintainers

摘要

The Triticum timopheevi cytoplasmic male sterility (cms) system in triticale (xTriticosecale Wittmack) suffers from a low frequency of maintainers and environmental instability of the male sterility. On the other hand, the Pampa cms system in rye (Secale cereale) exhibits strong male sterility and a low frequency of restorers. Here, we report generating hybrids between maintainers of the T timopheevi ems system in triticale and maintainers of the rye Pampa ems system. Ten hybrids were obtained. Their hybridity was verified by PCR (polymerase chain reaction) using ISSR (inter simple sequence repeats) primers. The ems maintaining ability of F-2 individuals and their progeny was tested. The F-2 plants were crossed to male sterile lines of triticale carrying the T timopheevi cytoplasm. Among 180 G(1) offspring of these crosses, 71(39.4%) were completely male sterile. Fourteen F-2 individuals (7.8%), as well as their F2S1 and progeny, generated stable male sterility in G(1), G(1)BC(1) and G(1)BC(2) generations after the crosses. Our results suggest that it is possible to produce a more stable ems system in triticale based on the T. timopheevi cytoplasm as compared to the existing one.