Structural and functional analysis of the 5′-flanking region of the human insulin-like growth factor binding protein (IGFBP)-4 gene

摘要

More than 3 kb of the human (h)IGFBP-4 gene 5′-flanking region was sequenced and assessed for promoter activity. The hIGFBP-4 promoter resides within a CpG island and demonstrates strong basal activity in human osteoblast-like osteosarcoma and COS-7 monkey kidney cells. Transient transfection of cells with hIGFBP-4 promoter-linked deletion constructs demonstrated that multiple cooperating cis-acting elements within 836 bp of the 5′-flanking region contributed to overall promoter strength.