Molecular characterization of kappa-casein gene inbuffaloes

摘要

The genetic variations in κ-casein gene locus exon IV of three buffalo breeds, i.e. South Kanara, Surti, andMurrah, were investigated. PCR amplification of the K-casein gene using primers specific for Bos taurus yielded DNAproducts of a similar size. Upon restriction digestion with Hinf I, two fragments having 266 bp and 84 bp were obtained forall the samples studied. Single strand conformation polymorphism (SSCP) and nucleotide sequence analysis also did notreveal genetic variation. BLAST alignment of 350 bp nucleotides of buffalo k-casein gene showed homogeneity with theκ-casein gene of Syncerus caffer nanus. Analysis of the κ-casein gene using PCR-restriction fragment length polymorphismand SSCP techniques including DNA sequencing data revealed a monomorphic pattern which is buffalo specific.