Polysaccharide-induced apoptosis in H22 cells through G2/M arrest and BCL2/BAX caspase-activated Fas pathway

摘要

The aim of the present work was to investigate the effect and the mechanism of growth inhibition on mouse H22 hepatocarcinoma cell of ascitic tumor induced by cartilage polysaccharides (PS). Our results showed that PS prolonged the survival time of the mice and increased the life span. In addition, PS induced the apoptosis of the H22 cells with the typical apoptotic morphological and biochemical changes confirmed by HE staining and TUNEL assay. The subsequent analysis of cell cycle distribution and relevant proteins revealed that decrease of cells in G0/G1phase and a G2/M arrest might due to the down-regulation of Cyclin D1 and AFP and up-regulation of P21 proteins. Moreover, BCL2/BAX caspase-activated Fas pathway was activated in PS-induced H22 apoptosis.