Culture of Staphylococcus xylosus in fish processing by-product-based media for lipase production

摘要

The objective of this study was to demonstrate that fish-processing by-products could be used as sole raw material to sustain the growth of Staphylococcus xylosus for lipase production. Bacterial growth was tested on supernatants generated by boiling (100pC for 20 min) of tuna, sardine, cuttlefish and shrimp by-products from fish processing industries. Among all samples tested, only supernatants generated from shrimp and cuttlefish by-products sustained the growth of S. xylosus. Shrimp-based medium gave the highest growth (A = 22) after 22 h of culture and exhibited the maximum lipase activity (28 U mlp#). This effect may be explained by better availability of nutrients, especially, in shrimp by-products. Standard medium (SM) amendments to sardine and tuna by-product-based media stimulated the growth of S. xylosus and the highest A values were obtained with 75% SM. Lipase activity, however, remained below 4 U mlp# for both sardine and tuna by-product-based media. Fish by-products could be used for the production of highly valuable enzymes. The use of fish by-products in producing S. xylosus-growth media can reduce environmental problems associated with waste disposal and, simultaneously, lower the cost of biomass and enzyme production.