Peg5/Neuronatin is an impaired gene located on sub-distal chromosome 2 in the mouse.

摘要

A systematic screen was established for imprinted genes using a subtraction-hybridization method with day 8.5 fertilized and parthenogenetic embryos. Two novel imprinted genes, Peg1/Mest and Peg3, have been identified previously by this method, along with the 2 known imprinted genes, Igf2 and Snrpn. Recently 3 additional candidate imprinted genes, Peg5-7, were detected and Peg5 was analysed further in this study. The cDNA sequence of Peg5 was identical to Neuronatin, a gene recently reported to beexpressed mainly in the brain. Two novel spliced forms were detected with some additional sequence in the middle of the known Neuronatin sequences. All alternatively spliced forms of Peg5 were expressed only from the paternal allele. This was confirmed using DNA polymorphism in a subinterspecific cross. Peg5/Neuronatin mapped to sub-distal chromosome 2, proximal to the previously established imprinted region where imprinted genes cause abnormal shape and behaviour in neonates.