ANTHER CULTURE OF PEPPER (Capsicum annuum L.): THE EFFECT OF NUTRIENT MEDIA

摘要

The development of valuable haploid plants useful in genetics as well as in practical selection is purpose of the in vitro cultivation of anthers. The first data for obtained haploids via anther culture of pepper (Capsicum annuum L.) were published byWang et al (1973), George et Narayanaswamy (1973) and Kuo ef al., (1973). The successful embryoid induction and microspore regeneration depends on a number of factors: stage of microspore development, low temperature pretreatment (Sibi ef al., 1979), elevated temperature treatments (Dumas de Vaulx et al., 1981), genotype and donor plant age (Morrison et al., 1986; Gomez & Chambonnet, 1992; Kristiansen & Andersen, 1993; Qin & Rotino, 1993; Ltifi & Wenzel, 1994; Mytiko et al., 1995), culture medium (Sibiet al., 1979; Vagera & Havranek, 1985; Pundeva et al., 1990; Boyaci, 2001; Ellialtioglu ef al., 2001) etc. The aim of the present study was to investigate in vitro response of anther culture of Bulgarian pepper lines, varieties and hybrids cultivated on different nutrient media.