Effects of Proteoglycan Extracted from Nasal Cartilage of Salmon Headson Maturation of Dendritic Cells Derived from Human Peripheral BloodMonocytes

摘要

Dendritic cells (DCs) play an essential role in the immune system. The transition from immature DC (iDCs)to mature DCs (mDCs) requires appropriate stimuli such as pro-inflammatory cytokines. Proteoglycans (PGs)are one of the main components of extracellular matrix, and some types of PGs are known to induce maturationof murine DCs. Recent studies have investigated the potential benefits of PG from nasal cartilage of salmon head(S-PG). This study investigated the effects of S-PG on maturation of human monocyte-derived DCs. iDCs wereprepared from human monocytes using the appropriate cytokines and then stimulated by S-PG alone. In anotherexperiment, iDCs were stimulated by a combination of pro-inflammatory cytokines (MIX) plus S-PG. Althoughthe stimulation of S-PG alone did not induce phenotypic maturation from iDCs, CD40 expression on DCs stimu-lated by S-PG alone was lower than that of iDCs. In contrast, the phenotypic and functional characteristics ofDCs stimulated by MIX+S-PG were similar to those of DCs stimulated by MIX alone. As a result, S-PG did notdemonstrate a significant effect with regard to maturation of human monocyte-derived DCs.