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首页> 外文期刊>Molecular ecology resources >Twenty-one novel microsatellite DNA loci isolated from the Puget Sound white-crowned sparrow, Zonotrichia leucophrys pugetensis
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Twenty-one novel microsatellite DNA loci isolated from the Puget Sound white-crowned sparrow, Zonotrichia leucophrys pugetensis

机译:从普吉特海湾白冠麻雀(Zonotrichia leucophrys pugetensis)分离出二十一个新的微卫星DNA基因座

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摘要

The white-crowned sparrow, Zonotrichia leucophrys, has served as a model species for studies of song and reproductive physiology. Here, we describe primers for 21 novel microsatellite loci isolated from the Puget Sound subspecies, Zonotrichia leucophrys pugetensis, which will be useful for parentage and population genetic analyses. Based on genotypes from seven to 22 adult birds from one population, the average number of alleles per locus was 10.9 (four to 21 alleles) and observed heterozygosity varied from 0.50 to 1.00. All loci also amplified products in at least one of three other passerine species tested.
机译:白冠麻雀(Zonotrichia leucophrys)已成为研究歌曲和生殖生理的典范。在这里,我们描述了21个从普吉特海湾亚种Zonotrichia leucophrys pugetensis分离出的新型微卫星基因座的引物,这将对亲子和种群遗传分析有用。根据来自一个种群的7至22只成年鸟的基因型,每个基因座的平均等位基因数量为10.9(4至21个等位基因),观察到的杂合度在0.50至1.00之间变化。所有基因座还扩增了至少三个其他受试雀形目物种之一的产物。

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