Failure in developing a model for complete vascular thrombosis in the common iliac artery in the rat.

摘要

The purpose of this study was to develop a model for complete arterial thrombosis proximal to the rat cremaster flap for subsequent fibrinolytic studies at the microcirculatory level. We divided 20 male Sprague-Dawley rats into four experimental groups of five animals each. We assigned each group to an established thrombosis model using crush and standard microsurgical anastomosis, crush and intimal abrasion, inverted arterial suture, and intravascular silk sutures combined with microsurgical anastomosis at the common iliac artery. Vessel patency was examined using the milking test 30, 60, 90, and 120 min after the thrombogenic insults. The model of perpendicular silk sutures and anastomosis caused complete arterial thrombosis in one animal over 120 min. The other models failed in all animals. In conclusion, the thrombogenic models used in this study are not capable of creating a reliable complete arterial thrombosis in the common iliac artery of the rat. Copyright 1999 Wiley-Liss, Inc.