Stimulatory Effect of Physicochemical Factors on the Expression of Cellulase by Trichoderma viride NSPRT23

摘要

Nutritional and environmental factors have a stimulatory effect on extracellular cellulases production. The present study dealt with the control of these factors with a view to trigger the biosynthesis of cellulases in submerged state fermentation. The process parameters optimized include; carbon sources, nitrogen sources, pH, incubation temperature, incubation time and substrate concentrations. Different fungal strains were screened for the production of cellulases in mineral salt medium in which carboxymethyl cellulose (CMC) had been incorporated as the sole carbon source. All the tested fungal strains produced cellulases with differences in the amount of cellulases produced. Of all the selected fungal isolates screened, Trichoderma viride NSPRT23was found to display highest cellulase activity. Among tested carbon sources, pineapple peels at a concentration of 5% was found to be the best carbon source for cellulases production. Different organic nitrogen sources were evaluated and cotton seeds were found to be the best for cellulases production. The optimum temperature, incubation time and pH for maximum cellulases production were 37°C, 72 h and 4.5, respectively.