Sample preparation steps for standardization of calcium ion determination in aerobically-treated sera accounting for influences of temperature and pH

摘要

A 5 min equilibration of 0.250 mL serum sample with room air containing 5.6% CO2, by placing the gas tube <1 cm above the sample-surface, enables a standardized determination of calcium ion concentration (cCa(2+)), referred to pH 7.40 and 37 degrees C, in aerobically handled specimens. Using this method, standardized measurement of calcium ions in serum is possible with a bias less than 2%, even in samples with pH-changes acquired during extended storage. [References: 11]