Molecular cloning, nucleotide sequence and structural analysis of the Streptomyces galbus DSM40480 fda gene: the S-galbus fructose-1,6-bisphosphate aldolase is a member of the class II aldolases

摘要

The fda gene of Streptomyces galbus DSM40480 encoding the fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) was cloned, sequenced and characterised. The fda gene encodes a protein of 341 amino acid with a molecular mass of 36.5 kDa and belongs to the class 11 aldolases. When the S. galbus fda gene was expressed in th Escherichia coli fda(ts) mutant NP315, the growth defect of the strain was complemented at temperatures >35 degreesC. IN Northern hybridisations, we identified in fda transcript of 1200 bp length. The transcript length indicates that the fda gene is transcribed from its own promoter. Attempts to isolate fda knock out mutants were not successful. Streptomyces lividans strains with a second copy of the fda gene were constructed and analysed. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. [References: 29]