CASE REPORT: EXCISION, AMNIOTIC MEMBRANE GRAFT, CRYOTHERAPY AND INTRALESIONAL MITOMYCIN C INJECTION FOR THE TREATMENT OF A LIMBAL MELANOMA IN A DOG

摘要

A four-year-old, female neutered, Shih-Tzu dog was presented with a one-year history of limbal masses on both eyes (OU, oculi utrque). The masses arose from the corneoscleral limbus with marked corneal involvement. An en bloc excision of the involvedmeridians with human amniotic membrane grafting was performed at the left eye (OS, oculus sinister) limbal masses. A double freeze-thaw cycles of cryotherapy and intralesional mitomycin-C (MMC) injection was performed on both eyes two weeks later. Histopathology provided the definitive diagnosis of limbal melanoma. After 6 months of follow-up, OU limbal masses recurred without intraocular invasion, and 2 cycles of cryotherapy and intralesional MMC injection was then performed on recurrent sites. Despitethat both cryotherapy and intralesional injection were applied the lesions recurred at an interval of 4-6 months for 18months. At the end only a small corneal scar was present on OS, and there was no recurrence for 3 years until now.The cryotherapy combined with intralesional MMC injection was able to control the recurrence of limbal melanoma after tumor excision while maintaining the ocular surface integrity.