Optimization of Enzyme Activity Assay for Screening of Acryl CoA: Monoacylglycerol Acyltransferase 2 Inhibitors

摘要

Acryl CoA: monoacylglycerol acyltransferase 2, (MGAT2) is interesting target and useful strategy for treating metabolic-related diseases such as obesity, type 2 diabetes, hypertriglyceridemia and non-alcoholic fatty liver disease. In this study, we are optimized a new and easy-to-use screening assay system to identify novel MGAT2 inhibitors. We optimized high-quality Perkinelmer's FlashPlates based MGAT2 assay have a short time-consuming, adjust for high throughput screening (HTS) and can perform extensive screening. This screening technology platform is considered to be particularly useful for biological targets for which the conventional HTS assays are difficult to implement.