Honey bee breeding is presently facing important challenges due to the need to breed bees that are tolerant or resistant to introduced or formerly unknown pathogens and parasites. At the same time, the diversity of the available gene pool is rapidly decreasing, with many subspecies and ecotypes of Apis mellifera facing massive introgression of foreign genotypes (De La Riia et al. 2009). The development of a practical means to store drone semen would enhance the bee breeder's ability to select and maintain superior honey bee stocks. A number of storage techniques have been tried with some success, using both ultra-cold freezing and non-frozen approaches (Collins 2000a).
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机译：由于需要育种对引入或以前未知的病原体和寄生虫具有耐受性或抗性的蜜蜂，因此蜜蜂育种目前面临重大挑战。同时，可用基因库的多样性正在迅速减少，蜜蜂亚种的许多亚种和生态型面临着外来基因型的大量渗入（De La Riia et al。2009）。开发一种存储无人蜂精液的实用方法将提高蜜蜂育种者选择和维持优质蜜蜂种群的能力。已尝试使用超冷冷冻和非冷冻方法进行许多存储技术的尝试（Collins 2000a）。
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