SummaryA method is described for the quantitation of disopyramide phosphate (NorpaceR in serum by high-performance liquid chromatography. The analysis utilizes ultraviolet detection at 254 nm with a 5-mu;m reversed-phase column and a mobile phase consisting of acetonitrile, ammonium dihydrogen phosphate buffer, acetic acid, and water. The determination requires 100 mu;l of serum sample and the analysis time (9 min) is rapid. Serum extraction is a two-step process using chloroform, sodium carbonate, and hydrochloric acid. Although acid is used, columns produced sharp peaks with consistent retention times for 6ndash;8 months. This method provides selectivity and sensitivity with a precision of 3.8percnt;, average recovery of 92.9percnt; and no interference from 18 commonly administered drugs.
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