Evaluation of Methods for Detecting the Production of H2S, Volatile Sulfides, and Greening by Lactobacilli

摘要

ABSTRACTThe ability to produce volatile sulfides by 41 lactobacilli was not associated with species but depended on the composition of the media and the sensitivity of the indicator. Only a restricted number ofL. plantarumstrains and seven isolates were H2S‐positive on peptone iron, TSI and SIM agars. Lead acetate agar detected a greater number of H2S‐positive lactobacilli but the lead acetate (or DTNB) paper method was superior in a modified motility sulfide broth. Cured meat agar detected the production of greening by H2CO2‐producing lactobacilli (L. fructovorans, L. jensenii, L. lactis, L. viridescens, F‐74, F‐99). Such tests, however, were not applicable to detect lactobacilli producing green sulf