Extended abstract:Chromosome rearrangements involved in transformation of human T‐Cell leukemia virus type I infected human lymphocytes

摘要

AbstractFollowing human T‐cell leukemia virus type I (HTLV‐I) infection, human lymphocytes (Coculture‐5) acquired capacity to grow continuously in the presence of cytokine. Coculture‐5 cells transformed (UV‐1) following ultraviolet (UV) irradiation. Coculture‐5 and UV‐1 cells had abnormalities of chromosomes 1 and 7. Coculture‐5 cells also transformed (Coculture‐15) following cocultivation with UV‐1 cells. In Coculture‐15, 18 structural abnormalities were observed. Of these, 6 were of chromosome 1 and 5 were of chromosome 7. The marker chromosome of UV‐1 cells, i(7q), was seen in up to 80 of Coculture‐15 cells. All UV‐1 cells examined had i(7q) with single centromeric G‐band, whereas 60 of Coculture‐15 cells had i(7q) with double centromeric G‐bands. By fluorescence in situ hybridization (FISH), an alpha satellite centromeric DNA probe for chromosome 7 gave a single band in i(7q) in UV‐1 and single or double bands in i(7q) in Coculture‐15 cells. These findings suggest that integration of HTLV‐I proviral DNA into chromosomal DNA causes fragility of certain chromosomes of infected cells and leads them to