The Use of RFLPs (Restriction Fragment Length Polymorphisms) Detects Germplasm Introgressions from Wild Species into Potato (Solanum tuberosumssp.tuberosum) Breeding Lines

摘要

AbstractThe germplasm of the potato (S. tuberosumssp.tuberosum) has been modified since the beginning of this century by breeders who introgressed important agronomic traits, for example disease resistance genes, from several wild and cultivatedSolanumspecies of the Americas. In this paper we show that the Rflpanalysis of potato‐breeding material can be used to detect chromosomal regions descended from more‐distantly relatedSolanumspecies. The Rflppatterns of individuals of tenSolanumspecies, ten breeding lines ofS. tuberosumssp.tuberosumand the cultivated variety ‘Bintje’ were analyzed. Rflpdata for each of eight single loci of known genomic position were used for the computation of locus‐specific phenograms by distance matrix methods. Several of the potato‐breeding lines deviated clearly from the clustered speciesS. tuberosum, S. stenotomumandS. canasensefor one or more of the loci considered. These deviations indicated the presence of “exotic” germplasm at a particular locus. The possibility of detecting such germplasm has implications for mapping the agronomic traits for which the wild species were introgressed and which may still be linked to “foreign” c