A new high sensitivity19F probe for labeling cysteine groups of proteins

摘要

AbstractA novel iodoacetamide label 4‐perfluoro‐tert‐butyl‐phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP;<100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40μM solution) can be detected easily in a single scan at