Results are presented which show that the Ames TDAreg; Theophylline kit provides an accurate and reliable method of measuring the concentration of theophylline in plasma. It may be used with a shortened incubation time (5 min) without loss of accuracy, and a single calibration curve can be used over a 3-week period. There is an acceptable precision at all assayed levels (coefficient of variation, 3.3percnt;) and results obtained for patients' samples correlate well with those obtained using liquid chromatography (y = 0.02 plus; l.06x, r = 0.99) and enzyme immunoassay (y = 0.58 plus; 1.03x, r = 0.99).
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