AbstractExperimental brain tumors produced in rats (n = 10) by stereotactic implantation of cells from the F98 anaplastic glioma clone into the right caudate nucleus were studiedin vivousing localized proton NMR andin vitrousing high‐resolution proton NMR, bioluminescent imaging of lactate, ATP and glucose distributions, and fluorescent imaging of regional pH.In vivospectra from normal brain contralateral to the tumor regions showed resonances assignable to N‐acetyl aspartate (NAA), creatines, choline‐containing compounds,myo‐inositol, glutamate and glucose in a pattern similar to those obtained from normal anaesthetized rats.In vivotumor spectra were characterized by the almost complete absence of NAA, a substantial reduction of total creatine and glucose, and an increase of cholines. Based on thein vitrospectra the increase of themyo‐inositol signal observedin vivowas mainly attributed to glycine. Histological examination as well as bioluminescent and fluorescent imaging indicated two stages of tumor development, i.e., solid vital tumors and tumors with necrosis. However, there was no consistent relationship between proton NMR observations and tumor de
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