Injectable nanohybrid scaffold for biopharmaceuticals delivery and soft tissue engineering

摘要

An injectable nanofibrous hydrogel scaffold integrated with growth factors (GFs) loaded polysaccharide nanoparticles was developed that specifically allows for targeted adipose-derived stem cells (ASCs) encapsulation and soft tissue engineering. The nanofibrous hydrogel was produced via biological conjugation of biotin-terminated star-shaped poly(ethylene glycol) (PEG-Biotin) and streptavidin-functionalized hyaluronic acid (HA-Streptavidin). The polysaccharide nanoparticles were noncovalently assembled via electrostatic interactions between low-molecular-weight heparin (LMWH) and N,N,N-trimethylchitosan chloride (TMC). Vascular endothelial growth factor (VEGF) was entrapped in the LMWH/TMC nanoparticles by affinity interactions with LMWH. A nanofibrous hydrogel is produced via the biological conjugation of biotin-terminated star poly(ethylene glycol) and streptavidin-functionalized hyaluronic acid. Vascular endothelial growth factor (VEGF) is entrapped in low-molecular-weight heparin/N,N,N-trimethylchitosan chloride nanoparticles by affinity interactions.