The Analysis of Clobazam and Its Metabolite Desmethylclobazam by Highhyphen;Performance Liquid Chromatography

摘要

SummaryThe analysis of clobazam and its metabolite desmethylclobazam by high-performance liquid chromatography is described. After adding an internal standard 500 mu;l of plasma is extracted under basic conditions into dichloroethane. The organic solvent is then evaporated to dryness and the residue reconstituted in 100 mu;l of mobile phase prior to injecting an aliquot (30 mu;l) onto a Hypersil 5 MOS column, which is eluted with acetonitrile/acetate buffer (pH 5.4) 40:60 vol/vol. The components are separated in approximately 12 min. Using this method, 15 mu; Lminus;1of clobazam and 30 mu; Lminus;1of desmethylclobazam can be detected. The method is suitable for the therapeutic monitoring of these two drugs in patient samples.