Chemical changes in the soluble carbohydrate and nitrogen fractions of grass and clover samples during freezing and thawing are described. Invertase activity was quickly evident in both grass and clover after their removal from cold storage. After 24 hr there was considerable loss of soluble carbohydrate in grass samples, but not in clover. The total nitrogen content of both grass and clover remained constant through to 60 hr after removal from cold storage, but extractable nitrogen was considerably reduced on freezing. The implications of these results to the freeze storage of experimental herbage diets in ruminant nutrition studies are discussed.
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