Preparation of a novel composite particles and its application in the fluorescent detection of proteins

摘要

A new fluorescence method for the detection of proteins with novel composite nanoparticles (CdS/PPA) has been developed. The composite nanoparticles have been prepared through an in-situ polymerization method under ultrasonic irradiation. The surface of the composite nanoparticles was covered with functional groups (-COOH). These groups may play a major role in the improving the water solubility and biocompatibility of the nanoparticles. The composite particles is combined with proteins in NaAc-HCl buffer solution (pH=1.99), which can result in strong fluorescence, and the response is linearly proportional to the concentration of proteins. In lambda em/lambda ex=650 nm/365 nm place (the stoke' shift is 285 nm), its fluorescent strength reaches the maximum. Under the optimum conditions, the linear range is 0.10-20.0 mu g center dot ml(-1) with the detection limit of 41 ng center dot ml(-1) for HSA, and 0.10-15.0 mu g center dot ml(-1) with the detection limit of 35 ng center dot ml(-1) for Human gamma-IgG . The method has been applied to the determination of the total protein in human serum samples collected from the hospital and the results are satisfactory.