Quantification of several monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine by high-performance liquid chromatography with fluorescence detection

摘要

A high-performance liquid chromatographic method with fluorescence detection has been developed to determine the urinary polycyclic aromatic hydrocarbon metabolites 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1-hydroxypyrene and 3-hydroxybenzapyrene. Solid phase extraction (SPE) was used to clean up the samples, and washing with 30 methanol was found to be the best way to remove interferences in the matrix. The method detection limits ranged from 0.044 mu g/L for 1-hydroxypyrene to 1.615 mu g/L for 3-hydroxybenzapyrene, and the recoveries ranged between 40 for 3-hydroxybenzapyrene and 99 for 2-hydroxynaphthalene. The within-day relative standard deviation was lowest for 2-hydroxynaphthalene at 0.67 and went up to 2.42 for 3-hydroxybenzapyrene, and the between-day relative standard deviation ranged from 3.84 for 9-hydroxyphenanthrene to 10.42 for 2-hydroxyfluorene. The correlation coefficients were between 0.9962 and 0.9998. The developed method was successfully used to analyze samples from student volunteers in a high school.