Localization of the catalytic subunit Cγ of the cAMP-dependent protein kinase gene (PRKACG) to human chromosome region 9q13

摘要

A cDNA for a new catalytic subunit (Cγ) of the cAMP-dependent protein kinase (PKA) was recently isolated from a human testis cDNA library. This subunit was shown to be expressed only in testis, and has so far not been demonstrated in other species. In the present study, we have determined the chromosomal localization of this gene employing a cDNA for Cγ as a probe. Southern blot analysis of genomic DNA from human × mouse somatic cell hybrids allowed us to assign this gene (PRKACG) to human chromosome 9. In situ hybridization to metaphase chromosomes confirmed the somatic cell hybrid data and regionally mapped the Cγ gene of PKA to human chromosome 9