Comparison of HPLC Separation of Vitamin D3Metabolites and Their Isotachysterol3Derivatives

摘要

Separation of vitamin D3, its four metabolites and their corresponding isotachysterol3derivatives was studied using four HPLC systems: two reverse phase columns, a silica column and a cyanopropyl silica column. Most of them gave a good separation between the compounds studied, although both reverse phase systems were less efficient in the separation of dihydroxylated vitamin D3metabolites and isotachysterol compounds. Isotachysterol3derivatives behaved analogously to their vitamin D3counterparts on all four systems, but their retention times were different. This indicates that chemical derivatization to isotachysterols can be used as a part of chromatographic identification of unknown vitamin D3metabolites as well as in the determination of major vitamin D3compounds in biological samples.