A near-diploid mouse ceil line has been produced from C57BL/6J X AKR/J F1-hybrid embryonic cells by spontaneous transformation. The modal chromosome number of this line is 41 (2n = 40), with low numerical and structural variation relative to other mouse lines which have been reported. Giemsa-banded karyotypes on a number of clones and subclones from this line have been analyzed. The modal karyotypes deviate very little from that of a normal diploid female. Most of the cells have two X chromosomes, one of which is unlabeled early in the S phase.
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