Rapid Separation of Prostaglandins by Linear High Performance Thin Layer Chromatography

摘要

A continuous development technique using silica gel linear high performance TLC plates is described for the separation of prostaglandins 6-keto-F1#x3B1;, F2#x3B1;, E2, 13,14-dihydro-15-keto-F2#x3B1;, 13#x2013;14-dihydro-15-keto-E2, and thromboxane B2. Complete separation of all six prostaglandins was achieved with a solvent system of ethyl acetate/acetone/acetic acid (90:5:1). The method is simple, rapid and provides excellent resolution of plasma prostaglandins prior to quantitation by gas chromatography-mass spectrometry.