Six fasting male mongrels served as pancreas donors. The pancreas was perfused without recirculation with a synthetic medium. The noradrenaline and adrenaline concentration in the efflux perfusate was determined by a double-isotope derivative technique. 1. The noradrenaline concentration in the efflux perfusate rose considerably (from 0.25 ng/ml to 10.0 ng/ml), when the pancreas was perfused with a glucose deprived perfusing medium. The concentration rose almost linearly with time. After the addition of very small amounts of glucose (2 mg/100 ml) to the perfusing medium there was a considerable decrease in catecholamine concentration and a further decrease with higher glucose concentrations. 2. No change in catecholamine concentration in the efflux perfusate was observed if the pancreas was perfused with a high glucose concentration during the whole experiment. 3. Glucagon release was also high during perfusion with a glucose deprived solution while insulin release was low. These experiments raise the question whether an increased catecholamine release may, at least partially, be responsible for the change in insulin and glucagon secretion during glucose deprivation.
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