Study DesignAn investigation of the use of intervetebral disc allografting as a potential alternative to intervertebral fusion was performed. In 13 adult mongrel dogs, intervertebral disc units cryopreserved after slow freezing were grafted.ObjectivesTo evaluate the morphologic and biosynthetic damage caused by cryopreserving the canine intervertebral disc before allografting.Summary of Background DataCryopreservation did not alter the normal histologic appearance. The metabolic activity, measured by35S-sulfate incorporation, was observed to be 44percnt; of the fresh control.MethodsTransplanted intervertebral discs were examined histologically and radiographically at 12, 24, and 48 weeks after surgery.ResultsThe allografted disc had preserved anular and nuclear architecture with moderate loss of chondrocytes 12 weeks after grafting. However, the intervertebral disc showed evidence of progressive disc degeneration 1 year after transplantation.ConclusionsMore investigation of storage methods in needed to enhance the viability of the intervertebral disc for successful intervertebral disc allografting.
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