A new sensitive method using solid-phase column extraction and high-performance liquid chromatography (HPLC) has been developed for the determination of lorazepam and its glucuronide metabolite in human plasma. Extraction was performed with a C2-Bond Elut column followed by reverse-phase HPLC of the sample. The detection limit of lorazepam in plasma was 0.5 mu;g/L. Standard curves over the concentration range from 2.5 to 75 mu;g/L had good linearity. Intra-assay variability ranged from 2.0 to 6.2percnt; and interassay variability from 4.8 to 8percnt; at the concentration range of 5ndash;50 mu;g/L.
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