Phase information, assumed known from three‐beam diffraction experiments, has been used successfully as input to direct methods to re‐determine the crystal structure of rubredoxin. With data at 1.54 Å resolution, starting sets containing 26 single phases, or alternatively 45 triplet phases, in both cases known with a mean error of ±22.5°, were sufficient to initiate solution of the structure. Conventional figures of merit were employed in the early stages to reject the majority of the incorrect phase models. The presence of a FeS4cluster in the structure was used in the interpretation of the initial maps. Phase sets including 2500E's with a mean single phase error 〈Δϕ〉≃ 70° or a mean triplet phase error 〈ΔΦ3〉≃ 80°, both relative to the model from the crystallographic refinement, could be refined and expanded by Fourier recycling using theSAYTANformalism. Several parameters have been varied to study their influence on phas
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