Extraction procedures of selenium compounds applied to solid biological samples (CRM 186: pig kidney, CRM 402: white clover and a selenium enriched yeast) are discussed. Enzymic hydrolysis led to high recoveries (57 to 92, depending on the sample). A hyphenated High Performance Liquid Chromatography-Electrothermal Atomic Absorption Spectrometry technique is used to study the speciation of selenium in the enzymic extracts. Organic compounds. selenomethionice are found as major components in these samples, 39, 56 and 77 for clover, kidney and yeast, respectively.
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