Cytometric and morphometric identification of diploid, tetraploid and pentaploid plants derived fromActinidia arguta(2n = 4x) crossed withA. deliciosa(2n = 6x)

摘要

AbstractInterspecific crosses betweenActinidia arguta(In = 4x) and A.deliciosa(2n = 6x) were carried out in order to transfer cold resistance from a wild to the cultivated kiwifruit species. Of the 65 progenies obtained and analysed by flow cytometry, 16 were diploid, one was tetraploid and 48 were, as expected, pentaploid. Morphologically, the diploids can be distinguished from the pentaploids by their longer lamina. The existence of 5x progenies confirms the existence of gene flow betweenA. argutaandA. deliciosa, whereas the existence of 2x and 4x progenies provides evidence for parthenogenesis within theActinidiagenus following an interspecific cross. The study demonstrates the utility of flow cytometry for genomic analysis of progenies derived from interspecific crosses. Possible uses of both sexual and parthenogenetic progenies in the breeding and genetics of kiwifruit are discussed.