THE SYNTHESIS OF CHALCONE COMPOUNDS WITH CL AND BR SUBSTITUENTS AND THEIR POTENTIAL ANTICANCER ACTIVITIES AGAINST MCF-7 BREAST CANCER CELLS

摘要

The synthesis of chalcone-based Cl and Br substituents has been successfully synthesized for determining the cytotoxic bioactivities against MCF-7 breast cancer cells.The method of synthesizing the chalcone compounds was carried out by Clasien-Schmidt condensation via aldol condensation aldol.The determination of chalcone chemical structures was conducted via elucidated structure.Characterizations of pure compounds were conducted via ~1H-NMR,FTIR,Mass spectrophotometer,and Spectrophotometry UV-VIS.The cytotoxicity behaviours were investigated via the addition of Prestoblue~TM reagents,while the IC50 score was also included as the parameter of investigation.Our findings show that three chalcone compounds yielded for 60.00,60.51,and 74.38 for(E)-3-(4-chlorophenyl)-1-o-tolilprop-2-en-1-on(1),(E)-1,3-bis(3-bromophenyl)prop-2-en-1-on(2),and(E)-3-(4-bromophenyl)-1-(3-chlorophenyl)prop-2-en-1-on(3)respectively.The synthesis results were in accordance with the target compounds,in which the characteristics of the chalcone compounds of C-Cl and C-Br were confirmed via FTIR spectra,~1H-NMR shows 8 signals which equal to 10 hydrogen atoms,indicated.The cytotoxic activities based on IC50 for chalcones 1,2,and 3 were 3.66 μg/mL,51.05 μg/mL,and 21.62 μg/mL respectively with the potential for anti-breast cancer via MCF-7 cells via the morphological changes.