EFFECT OF BACTERIA INOCULATION AND IRON APPLICATION ON NITROGEN FIXATION IN SOYBEAN IN COMMON SOIL SERIES OF AMIK PLAIN (HATAY, TURKEY)

摘要

In this research, the effects of bacteria inoculation and iron application on the soybean plant, via pot experiments, using common soil series belonging to Aktas, Akkerpic, Akkuyu, Asagioba and Asi in the Amik Plain, were investigated. The experimental design consisted of two bacteria (B0: without inoculation and B1: bacteria strain nr. 110), and two iron (Fe0: without iron and Fe1: 5 mg kg-1 Fe) on selected five soil series (S1: Aktas S2: Akkerpic; S3: Akkuyu; S4: Asagioba; S5: Asi) with three replications. Plant and soil samples were taken during the blooming period. Nodulation, biomass development and the nitrogen content were analyzed in plant samples, whereas the respiration (C02 production), dehydrogenase enzyme activity (DHA) and microbial biomass carbon (MBC) contents of the soil have been determined. The number of nodules were 4, 12, 12, 4 and 0 number.plant1 in Aktas, Akkerpic, Akkuyu, Asagioba and Asi, respectively. The nodule formation was not observed in non-inoculated pots. Within the inoculated applications, the plant was not developed in Asi series, therefore the nodule was not formed. In other soil series an average of five nodules per plant were determined. Bacterial inoculation and iron application together increased the nodule number up to 20 nodules per plant. The determined DHA, CO2 and MBC results were 110 g TPF 10 gds1, 15.2 mg C 100 gds1. 24 h 1 and 9.42 mg C 100 gds1 in bacteria inoculated variant while the values in non-inoculated plants were 90 g TPF 10 gds1, 14.2 mg C 100 gds" 1, 24 h-1, 7,93 mg C 100 gds1, respectively. According to the general average results, bacterial inoculation and iron application have positive effects on nodule formation and nitrogen fixation in the common soil series of Amik Plain.