Analysis of Dynamic Processes in Biological Systems Using Acousto-Optic Video Spectrometry

摘要

A measurement setup based on a transmission microscope with double acousto-optical wavelength selection is proposed for detection of high-quality spectral images with a size of 1920 x 1200 pixels in the visible wavelength range with a spectral resolution of 2.5 nm at a wavelength of 632 nm. The efficiency of the proposed approach and setup is demonstrated in the study of the transition ofMisgurnus fossilisembryos from the 32nd to the 33rd stage. Correctness of the conclusions is proven in a comparison with the results of the corresponding ultrasonic study. Analysis of time dependences of the transmission spectra is performed for six spatial regions of the embryo including perivitelline space and head and tail sections. Such substantially different dependences can be used, in particular, for noninvasive monitoring and analysis of changes in the functional state of embryos related to the stage-to-stage transition.