Piezoelectric MoS2 Nanoflowers (NF's) for Targeted Cancer Therapy by Gelatin-based Shear Thinning Hydrogels. In vitro and In vivo trials

摘要

Cancer is a lethal disease found worldwide, however, unmet clinical needs demand the development of robust anticancer therapeutics The efficacy of injectable shear-thinning biomaterials for diseases such as cancer requires stability, interaction with the host tissue, and real-time tracking of the biomaterial. Here we report, molybdenum disulfide (MoS2) Nanoflowers (NFs) injected with stable, non-invasive, novel physically crosslinked injectable shear-thinning biomaterial named 6NC50, prepared by 50 wt of gelatin, and silicate nanoplatelets. The 6NC50-MoS2 formulations were assessed in terms of structure, injectability, and rheological stability. Cytotoxicity profile of 6NC50-MoS2, against NIH3T3 under 3 mins ultrasonication to induce piezoelectric properties of MoS2, showed that ultrasonication and direct contact of STB with the cells were effective for cell death for 7 days. In vitro cytotoxicity against B16F10 with 3 mins of ultrasonication and direct contact showed that 6NC50-M3 and 6NC50-M5 having 3 and 5 mg/mL MoS2 have 80-90 cell death in 7 days. In vivo monitoring of MoS2-loaded 6NC50 on mouse models by tumor imaging, tumor volume, and histopathology showed that 6NC50-M5 incor-porated mice showed 84 tumor reduction in 7 days.