Effect of different concentrations of Taurine on certain Physico-morphological attributes during cryopreservation of Gir bull semen

摘要

The study was carried out on four Gir bull (4-6 years) located at Cattle Breeding Farm J.A.U. Junagadh for a period of 6 weeks. Semen was collected using artificial vagina method once weekly for six weeks from each bull. The semen was evaluated for individual motility, sperm viability and sperm abnormality at pre-freeze and post-thaw stages of cryopreservation using different Taurine concentrations viz. 25 mM taurine, 50 mM taurine, 75 mM taurine and control (without taurine). At pre -freeze stages of cryopreservation, 50 mM taurine group had value of 70.20 +/- 1.22 and 69.87 +/- 3.09 percent for individual motility and sperm viability respectively, values were significantly (P<0.05) higher as compared to that of the 25 mM taurine, 75 mM taurine and control groups. Mean per cent sperm abnormality was 15.37 +/- 0.89 in 50mM taurine group at pre-freeze stages of cryopreservation, which were significantly (P<0.05) lower as compared to that of the other groups. At post-thaw stages of cryopreservation, 50 mM taurine group had values of 62.70 +/- 0.42 and 65.66 +/- 0.58 percent for individual motility and sperm viability respectively, the values were significantly (P<0.05) higher as compared to that of the 25 mM Taurine, 75 mM Taurine and control groups. Mean per cent sperm abnormality was 20.29 +/- 0.73 in 50mM Taurine group at post-thaw stages of cryopreservation, which were significantly (P<0.05) lower as compared to that of the other groups. Individual motility and sperm viability was found to be significantly better in 50 mM taurine group at pre-freeze and post-thaw stages of cryopreservation of Gir bull semen.