THE INHIBITORY ON LIPASE ACTIVITY AND LD50 DETERMINATION OF HERBAL FORTIFIED GREEN TEA(GAMBOENG TEA)

摘要

A randomized controlled trial was conducted on three formulas of an herbal drink containing green"gamboeng"tea fortified with bay leaf and cinnamon bark at various concentrations.One gram of mixed formula was brewed in hot water at 70℃-90 ℃ for 10-15 minutes,and the yield of each plant was determined.We also determined the phenolic content and lipase inhibition activity of each formula.We obtained a yield of each plant with green"gamboeng"tea(66.2 %),bay leaves(10.7 %),and cinnamon bark(44.8 %).Green"gamboeng"tea has the highest percentage of phenolic compound 30.25 ± 1.201 %.Inhibition lipase activity was calculated using an Elisa reader at X =405 nm.We have three formulas with the highest lipase inhibition activity.For the three formulas,a mixture of green"gamboeng"tea,bay leaf,and cinnamon bark was F-3.2(80.598%),F-4.1(74.306),and F-5.2(80.299%).We also conducted acute toxicity for the formulas above using rats as an animal model.We observed the body weight,organ weight,motoric,and mortality of rats during 14 days of observation,after using the formula with the concentration of 3.75,7.5,and 15 g/kg body weight.We also used a control group of rats in this experiment.The results of acute toxicity showed no mortality of rats from the lowest to the highest doses.We concluded that LD50 of the three formulas was>15 g/kg body weight.Between the treated groups and the control groups for organ weight and motoric activity showed no significant difference.Our conclusion is that this herbal formula drink has an activity for lipase inhibition,and is also safe for consumption by humans that suffer from obesity.